Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, ProClin 300.
Expression System:
E. coli
Form:
Lyophilized powder
Sequence:
N-terminal His-Tag, Gly31~Arg286 (NP_067431.2)
Application Notes:
arboxylesterase 1 (CES1) also known as liver carboxylesterase 1 is a serine esterase and member of a large multigene carboxylesterase family. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant mouse CES1 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris (pH 7.5, the Assay Buffer), ainitiated by addition 50 µl of various concentrations of CES1 (dilute by Assay Buffer) to 50 µl of 2 mM Substrate 4-NPA (100 mM stock in Acetone, dilute by deionized water). Incubated at 37C for 10min, then read at a wavelength of 400nm. One unit of enzyme activity is defined as the 1 µg of enzyme required to convert 1 pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37C. The specific activity of recombinant mouse CES1 is 1648 pmol/min/µg.
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