Mouse SCF protein, His tag (active), Unconjugated

Product Overview

• Article Name: Mouse SCF protein, His tag (active), Unconjugated
• Biozol Catalog Number: GTX00323-PRO
• Supplier Catalog Number: GTX00323-pro
• Alternative Catalog Number: GTX00323-PRO-10
• Manufacturer: GeneTex
• Category: Proteine/Peptide
• Application: FA
• Species Reactivity: Mouse
• Conjugation: Unconjugated
• Alternative Names: kit ligand , Clo , Con , Gb , Kitlg , Mgf , SCF , SF , SLF , Sl , blz , contrasted

Product Properties

• NCBI: 17311
• UniProt: P20826
• Buffer: Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.4), 0.01% SKL, 1mM DTT, 5% Trehalose, ProClin 300.
• Expression System: E. coli
• Form: Lyophilized powder
• Sequence: N-terminal His-Tag, Lys24~Ala219 (NP_001334085.1)

Application Notes

• Application Notes: Stem cell factor (also known as SCF, KIT-ligand, KL, or steel factor) is a cytokine that binds to the c-KIT receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis. SCF has been shown to stimulate the proliferation of Raw264. 7 cells. To test this effect, Raw264. 7 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach later, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM containing various concentrations of recombinant mouse SCF. After incubated for 120h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37C. Proliferation of Raw264. 7 cells after incubation with SCF for 120h observed by inverted microscope.