20mM HEPES, 80mM NaCl, 10% Glycerol, no preservative.
Expression System:
E. coli
Purity:
Multi-Step Purified
Form:
Liquid
Sequence:
Full-length protein, NP_036243.1 with N-terminal SH residue
Application Notes:
2 µM AHA1 Protein generated a 9-fold ATPase activation of 2 µM HSP90 (His-tagged HSP90 beta) in 33 mM HEPES (pH 7.2) with 30 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1.5 mM ATP in a 100 µl reaction at 37 C. (This is an enzyme-linked ATP regeneration assay tracking loss of NADH absorbance at 340nm.)
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