NUP98 antibody [21A10], IgG1, Unconjugated, Mouse, Monoclonal

Article Name:	NUP98 antibody [21A10], IgG1, Unconjugated, Mouse, Monoclonal
Biozol Catalog Number:	GTX00695
Supplier Catalog Number:	GTX00695
Alternative Catalog Number:	GTX00695-100
Manufacturer:	GeneTex
Host:	Mouse
Category:	Antikörper
Application:	ICC, WB
Species Reactivity:	Fungi, Human, Mouse, Other, Yeast
Immunogen:	Synthetic peptides containing conserved N-terminal sequence, GLFG, of Nup98 protein of Tetrahymena thermophila. Peptide 1, 1-MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ-29 Peptide 2, 646-SNPTQGGGLFGAANPGLGG-664 Epitope determined: GLF
Conjugation:	Unconjugated
Alternative Names:	nucleoporin 98 , ADIR2 , NUP196 , NUP96

Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and

Clonality:	Monoclonal
Concentration:	1 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation:	[21A10]
Molecular Weight:	198
Isotype:	IgG1
Sensitivity:	For WB application, it works on S. cerevisiae and S. pombe nut may be not suitbale for human, T. etrahymena and yeasts. Recommend GTX00693 NUP98 antibody [13C2] on human, T. etrahymena and yeasts samples.
NCBI:	4928
Pubmed:	23607342,
UniProt:	P52948
Buffer:	Filter-sterilized PBS, 50% Glycerol, no preservative.
Source:	Tetrahymena
Purity:	Purified IgG
Form:	Liquid

Application Notes:

*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

	Summary of the suitability of GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10] for immunological applications.
	WB analysis of S. cereviciae celll extracts using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. Dilution : 13C2 or 21A10 : 1:10 2H10 : 20 μg/ml
	GTX00695 ICC/IF Image
	WB analysis of S. pombe cell l extracts using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. Left and right lanes represent specimens from a wild type strain and an S. pombe strain in which Nup98 was chromosomally replaced with Nup98-GFP, respectively. Dilution : 13C2 or 21A10 : 1:10 2H10 : 1 μg/ml
	WB analysis of Tetrahymena themophila cells or Tetrahymena themophila cells overexpressing GFP-MacNup98A using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. Diamonds and asterisks represent uncharacterized proteins.
	ICC/IF analysis of Tetrahymena themophila cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. 13C2 mAb was highly specific to the macronucleus. In contrast, in addition to clear macronuclear staining, 21A10 mAb also stained the micronuclear periphery. This indicates that 21A10 mAb recognizes Nups localizing to the micronucleus such as Nup308 in addition to MacNup98A. Neither mABs 2H10 nor 414 could stain nuclear periphery of Tetrahymena. Green : Primary antibody Violet : DAPI Dilution : 0.5 μg/ml Fixation : Cold Methanol (-30 degree C) for 30 min
	WB analysis of HeLa whole cell lysate using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. Dilution : 0.4 μg/ml
	ICC/IF analysis of S. cereviciae cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. Green : Primary antibody Violet : DAPI Dilution : 13C2 or 21A10 : 1:10 2H10 : 10 μg/ml
	ICC/IF analysis of HeLa cells using GTX00693 NUP98 antibody [13C2], GTX00695 NUP98 antibody [21A10], or GTX00697 NUP98 antibody [2H10]. The signal at the nuclear periphery with 21A10 mAb was much higher and the background lower than that of 2H10 and 13C2 antibodies. Green : Primary antibody Violet : DAPI Dilution : 0.5 μg/ml Fixation : Cold Methanol (-30 degree C) for 30 min