The Hit3a antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor - CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.The Hit3a antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of Hit3a antibody to CD3e can induce cell activation. The antibody has also been demonstrated to be cross-reactive with Chimpanzee CD3.
Clonality:
Monoclonal
Concentration:
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
FCM: 0.5 µg (5 µl) for 105-108 cells in 100 µl sample per test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
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