ConA Lectin (Biotin)

Product Overview

• Article Name: ConA Lectin (Biotin)
• Biozol Catalog Number: GTX01504
• Supplier Catalog Number: GTX01504
• Alternative Catalog Number: GTX01504-1
• Manufacturer: GeneTex
• Category: Proteine/Peptide
• Application: ELISA, IHC, WB
• Species Reactivity: Plant
• Conjugation: Biotin
• Alternative Names: Concanavalin A , Concanavalin A , Canavalia ensiformis , Canavalia ensiformis lectin , ConA lectin , Canavalia ensiformis , ConA , Concanavalin A lectin , Con A lectin

Product Description

Concanavalin A is a lectin protein (MW 104kDa), homotetramer 26 kDa, originally extracted from the jack-bean, Canavalia ensiformis. It binds specifically to certain structures found in various sugars alpha-mannosyl and alpha-glucosyl residues in glycoproteins. It was the first lectin to be available on a commercial basis and is widely used in biology and biochemistry to characterize glycoproteins and other sugar-containing entities. It is also used to purify macromolecules in lectin affinity chromatography. Concanavalin A interacts with diverse receptors containing mannose carbohydrates (serum and membrane glycoproteins).ConA agglutinate strongly erythrocytes without being blood group specific. Normal cell re agglutinated after trypsinisation. ConA is not also a lymphocyte mitogen and reacts with many bacteria, like E. coli Dictyostelium discoideum et B. substilis, but also widely believed to be involved in the interaction between alpha-mannosyl oligosaccharides on the surface of the HIV virus and the human T cell lymphocyte.

Product Properties

• Concentration: 5 mg/ml (Please refer to the vial label for the specific concentration.)
• Sensitivity: alpha-linked mannose and glucose
• Buffer: 10mM Bicarbonate, 150mM NaCl, 0.1mM CaCl2, 0.01mM MgCl2 (Con A has an Isoelectric point of about pH4.5-5.5, requires calcium or manganese ions at each of its four saccharide binding sites. These ions should be used in buffer. DO NOT USE Phosphate buffer f
• Expression System: Native Protein
• Form: Liquid
• Sequence: Native Protein

Application Notes

• Application Notes: WB: 1:500-1:2000. ELISA: 1:500-1:2500. IHC: 1:250-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.