SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active), Unconjugated

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active), Unconjugated
• Biozol Catalog Number: GTX02575-PRO
• Supplier Catalog Number: GTX02575-pro
• Alternative Catalog Number: GTX02575-PRO-100
• Manufacturer: GeneTex
• Category: Proteine/Peptide
• Application: ELISA, FA
• Species Reactivity: Virus
• Conjugation: Unconjugated
• Alternative Names: COVID-19 Spike, SARS-CoV-2 Spike, covid-19, sars-cov-2

Product Description

SARS-CoV-2 (COVID-19) Spike (D614G Mutant)(ECD) protein, His tag (active), Unconjugated

Product Properties

• Concentration: Batch dependent (Please refer to the vial label for the specific concentration.)
• NCBI: 43740568
• Buffer: PBS, 20% Glycerol, no preservative.
• Expression System: HEK293 cells
• Purity: Purified by affinity chromatography
• Form: Liquid
• Sequence: C-terminal 8x His tagged, 16-1213 a.a. (Uniprot P0DTC2, D614G variant). Full-length soluble with foldon trimerization motif, mutated Furin recognition site and 3 stabilising mutations (F817P, K986P and V987P), based on/modified from Hsieh et al, 2020.

Images

GTX02575-pro Functional Assay Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (D614G variant) protein, His tag (active) (GTX02575-pro), and SARS-CoV-2 spike (trimer) protein using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 μg/mL.

SDS-PAGE of GTX02575-pro SARS-CoV-2 (COVID-19) Spike (D614G variant) protein, His tag.

CD spectra of different cation forms of spike protein. The scanning fluorimetry data of the two batches shows the protein is both stable and functional, with heparin binding activity.
A and C : CD spectra were recorded on J-1100 spectrometer between 180 and 260 nm of spike protein (0.5 mg/ml).
B and D : Secondary structure were analysed by program CDSSTR of A and C, respectively.
A and B : the first batch of spike protein (PBS buffer).
C and D : the second batch of spike protein (PBS, 20% glycerol).

Gel filtration analysis of 2 different batches of spike protein.
The first batch : spike protein in PBS buffer.
The second batch : spike protein in PBS, 20% glycerol.

Differential scanning fluorimetry (DFS) analysis the stability and the heparin binding ability. DSF of proteins were performed in the absence or presence of heparin (10 μM). The scanning fluorimetry data of the two batches shows the protein is both stable and functional, with heparin binding activity.
A and C : Melting curve of spike protein.
B and D : First derivative of the melting curves of spike to show its melting temperature as peak.
A and B : the first batch of spike protein (PBS)
C and D : the second batch of spike protein (PBS, 20% glycerol).