Human Antithrombin III protein (active), Unconjugated
Biozol Catalog Number:
GTX05084-PRO
Supplier Catalog Number:
GTX05084-pro
Alternative Catalog Number:
GTX05084-PRO-10
Manufacturer:
GeneTex
Category:
Proteine/Peptide
Application:
FA
Species Reactivity:
Human
Conjugation:
Unconjugated
The activity of recombinant human antithrombin III was measured by its ability to inhibit thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Thrombin was diluted to 0.5 U with heparin at 50 µg/ml in the assay buffer and 10 ul different concentrations of recombinant human antithrombin III (MW: 50 KD) was incubated with 10 ul diluted thrombin at 37 C for 30 minutes. Loading 50 µL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 µL of 200 µM substrate. Include a substrate blank containing 50 µL of assay buffer and 50 µL of 200 µM substrate. Then read at excitiation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes. This recombinant human antithrombin III significantly decreased thrombin activity. The inhibition IC50 was <8 nM.
Buffer:
Reconstitute with 10mM PBS to 0.1-1.0 mg/mL. Do not vortex. Lyophilized from PBS, 0.01% SKL, 5% Trehalose, no preservatives.
Expression System:
Native protein
Form:
Lyophilized powder
Sequence:
Native human Antithrombin III protein
Application Notes:
The recombinant human antithrombin III significantly decreased thrombin activity. The inhibition IC50 was <8 nM.
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