DDB1 antibody [C3], C-term, IgG, Clone: Polyclonal, Unconjugated, Human

Catalog Number: GTX100232
Article Name: DDB1 antibody [C3], C-term, IgG, Clone: Polyclonal, Unconjugated, Human
Biozol Catalog Number: GTX100232
Supplier Catalog Number: GTX100232
Alternative Catalog Number: GTX100232-100,GTX100232-25
Manufacturer: GeneTex
Host: Human
Category: Antikörper
Application: WB, IHC-P, ICC
Species Reactivity: Human, Mouse, Rat
Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DDB1. The exact sequence is proprietary.
Conjugation: Unconjugated
Alternative Names: damage specific DNA binding protein 1 , DDBA , UV-DDB1 , XAP1 , XPCE , XPE , XPE-BF
Clonality: Polyclonal
Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation: Polyclonal
Molecular Weight: 127
Isotype: IgG
NCBI: 1642
UniProt: Q16531
Buffer: 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purity: Purified by antigen-affinity chromatography.
DDB1 antibody [C3], C-term detects DDB1 protein at cytosol and nucleus on mouse heart by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
DDB1 antibody [C3], C-term (GTX100232) dilution: 1:500.
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Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DDB1 antibody [C3], C-term (GTX100232) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
DDB1 antibody [C3], C-term detects DDB1 protein at cytosol and nucleus on mouse hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hind brain.
DDB1 antibody [C3], C-term (GTX100232) dilution: 1:500.
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Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
DDB1 antibody [C3], C-term detects DDB1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DDB1 protein stained by DDB1 antibody [C3], C-term (GTX100232) diluted at 1:500.
Blue: Hoechst 33343 staining.
Sample (30 μg of whole cell lysate)
A: Neuro2A
B: GL261
C: C8D30
7.5% SDS PAGE
GTX100232 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunohistochemical analysis of paraffin-embedded U373 xenograft, using DDB1(GTX100232) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (30 μg of whole cell lysate)
A: PC-12
7.5% SDS PAGE
GTX100232 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (30 μg of whole cell lysate)
A: A549
B: H1299
C: HCT116
D: MCF-7
7.5% SDS PAGE
GTX100232 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
DDB1 antibody [C3], C-term detects DDB1 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
DDB1 antibody [C3], C-term (GTX100232) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min