SOD1 antibody, IgG, Clone: Polyclonal, Unconjugated, Human

Catalog Number: GTX100554
Article Name: SOD1 antibody, IgG, Clone: Polyclonal, Unconjugated, Human
Biozol Catalog Number: GTX100554
Supplier Catalog Number: GTX100554
Alternative Catalog Number: GTX100554-100,GTX100554-25
Manufacturer: GeneTex
Host: Human
Category: Antikörper
Application: WB, IHC-P, ELISA, ICC
Species Reactivity: Mouse, Human, Reptile, Aves, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human SOD1. The exact sequence is proprietary.
Conjugation: Unconjugated
Alternative Names: superoxide dismutase 1 , ALS , ALS1 , HEL-S-44 , IPOA , SOD , hSod1 , homodimer
Clonality: Polyclonal
Concentration: 0.15 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation: Polyclonal
Molecular Weight: 16
Isotype: IgG
NCBI: 6647
UniProt: P00441
Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Purity: Purified by antigen-affinity chromatography.
SOD1 antibody detects SOD1 protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
SOD1 antibody (GTX100554) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Sample (50 μg of whole cell lysate)
A: mouse brain
15% SDS PAGE
GTX100554 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100554) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (50 μg of whole cell lysate)
A: Rat brain
15% SDS PAGE
GTX100554 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SOD1 antibody detects SOD1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100554) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunofluorescence photomicrographs of paraffin-embedded mouse fetal brain.
Green: SOD1 antibody (GTX100554) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).
Red: beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:100. The signal was developed using goat anti-mouse IgG antibody (Dylight594) (GTX213111-05).
Blue: Nuclear staining with Hoechst 33342.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
ELISA detection of SOD1 using GTX100554 for capture at a concentration of 5 μg/mL and GTX89049 for detection at a concentration of 1.5 μg/mL.
SOD1 antibody detects SOD1 protein at nucleus by immunofluorescent analysis.
Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SOD1 protein stained by SOD1 antibody (GTX100554) diluted at 1:500.
Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody (GTX100554) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.