Annexin A1 antibody, IgG, Clone: Polyclonal, Unconjugated, Human

Catalog Number: GTX101070
Article Name: Annexin A1 antibody, IgG, Clone: Polyclonal, Unconjugated, Human
Biozol Catalog Number: GTX101070
Supplier Catalog Number: GTX101070
Alternative Catalog Number: GTX101070-100,GTX101070-25
Manufacturer: GeneTex
Host: Human
Category: Antikörper
Application: WB, IHC-P, ICC, IP
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary.
Conjugation: Unconjugated
Alternative Names: annexin A1 , ANX1 , LPC1
Clonality: Polyclonal
Concentration: 0.94 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation: Polyclonal
Molecular Weight: 39
Isotype: IgG
NCBI: 301
UniProt: P04083
Buffer: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Purity: Purified by antigen-affinity chromatography.
Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
10% SDS PAGE
GTX101070 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunoprecipitation of Annexin A1 protein from HeLa whole cell extracts using 5 μg of Annexin A1 antibody (GTX101070).
Western blot analysis was performed using Annexin A1 antibody (GTX101070).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Immunohistochemical analysis of paraffin-embedded N87 Xenograft , using Annexin I(GTX101070) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Annexin A1 antibody (GTX101070) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.
Annexin A1 antibody detects Annexin A1 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Annexin A1 stained by Annexin A1 antibody (GTX101070) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.
Annexin A1 antibody detects Annexin A1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Annexin A1 stained by Annexin A1 antibody (GTX101070) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min