Annexin A1 antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Annexin A1 antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX101070
• Supplier Catalog Number: GTX101070
• Alternative Catalog Number: GTX101070-100,GTX101070-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC-P, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: annexin A1 , ANX1 , LPC1

Product Properties

• Clonality: Polyclonal
• Concentration: 0.95 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 39
• NCBI: 301
• UniProt: P04083
• Buffer: 1XPBS (pH7), 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX101070 IHC-P Image

Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: NIH-3T3
B: JC
10% SDS PAGE
GTX101070 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of Annexin A1 protein from HeLa whole cell extracts using 5 μg of Annexin A1 antibody (GTX101070).
Western blot analysis was performed using Annexin A1 antibody (GTX101070).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemical analysis of paraffin-embedded N87 Xenograft , using Annexin I(GTX101070) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Annexin A1 antibody (GTX101070) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Annexin A1 antibody detects Annexin A1 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Annexin A1 stained by Annexin A1 antibody (GTX101070) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.