WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX101147 ELISA Image
Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm on human renal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human renal carcinoma . Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at endoplasmic reticulum by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:2000.Blue: Hoechst 33342 staining.Scale bar= 10μm.
Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat spleen.Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Wild-type (WT) and Heme Oxygenase 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody (GTX101147) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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