Oct4 antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Oct4 antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX101497
• Supplier Catalog Number: GTX101497
• Alternative Catalog Number: GTX101497-100,GTX101497-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: FACS, ICC, IHC-Fr, IHC-P, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Oct4. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: POU class 5 homeobox 1 , OCT3 , OCT4 , OTF-3 , OTF3 , OTF4 , Oct-3 , Oct-4

Product Properties

• Clonality: Polyclonal
• Concentration: 2.55 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 39
• NCBI: 5460
• UniProt: Q01860
• Buffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:5000-1:100000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX101497 WB Image

Immunofluorescence analysis of hESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.

Immunofluorescence analysis of mESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.

Sample (20 μg of whole cell lysate)
A: mouse ESC
12% SDS PAGE
GTX101497 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX101497) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX101497) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Oct4 antibody detects Oct4 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Oct4 protein stained by Oct4 antibody (GTX101497) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Oct4 antibody detects Oct4 protein by western blot analysis.
A. 30 μg human ESC whole cell extract
12% SDS-PAGE
Oct4 antibody (GTX101497) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:100000.

Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX101497)antibody at 1:100 dilution(purple) or rabbit IgG (black).

Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Oct4 stained by Oct4 antibody (GTX101497) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min