1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Purified by antigen-affinity chromatography.
Oct4 antibody detects Oct4 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Oct4 stained by Oct4 antibody (GTX101497) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Oct4 antibody (GTX101497) diluted at 1:100000.
Flow cytometry on human embryonic stem cells, staining with Oct4 (GTX101497)antibody at 1:100 dilution(purple) or rabbit IgG (black).
Immunofluorescence analysis of hESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.
Immunofluorescence analysis of mESCs on CF1 feeders, using OCT3/4(GTX101497) antibody at 1:200 dilution.
Sample (20 μg of whole cell lysate) A: mouse ESC 12% SDS PAGE GTX101497 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Oct4 antibody immunoprecipitates Oct4 protein in IP experiments. IP Sample: cell lysate/extract of Oct4 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Oct4 by 2 μg of Oct4 antibody (GTX101497) 12% SDS-PAGE The immunoprecipitated Oct4 protein was detected by Oct4 antibody (GTX101497) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Oct4 antibody detects Oct4 protein at nucleus by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Oct4 protein stained by Oct4 antibody (GTX101497) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Oct4 antibody detects Oct4 protein by western blot analysis. A. 30 μg human ESC whole cell extract 12% SDS-PAGE Oct4 antibody (GTX101497) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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