GAD67 antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: GAD67 antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX101881
• Supplier Catalog Number: GTX101881
• Alternative Catalog Number: GTX101881-100,GTX101881-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human GAD67. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: glutamate decarboxylase 1 , CPSQ1 , GAD , SCP

Product Properties

• Clonality: Polyclonal
• Concentration: 0.22 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 67
• Sensitivity: This antibody is specific for GAD67 protein, and it does not cross-react with GAD65 protein.
• NCBI: 2571
• UniProt: Q99259
• Buffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX101881 WB Image

GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD67 antibody detects GAD67 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD67 antibody (GTX101881) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GAD67 antibody detects GAD67 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human GAD1-transfected 293T cells
7.5% SDS-PAGE
GAD67 antibody (GTX101881) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GAD67 antibody detects GAD67 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GAD67 antibody detects GAD67 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GAD67 stained by GAD67 antibody (GTX101881) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min