GOT2 antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: GOT2 antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX101929
• Supplier Catalog Number: GTX101929
• Alternative Catalog Number: GTX101929-100,GTX101929-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the center region of human GOT2. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: glutamic-oxaloacetic transaminase 2 , KAT4 , KATIV , KYAT4 , mitAAT

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 48
• NCBI: 2806
• UniProt: P00505
• Buffer: 1XPBS (pH7), 1% BSA, 20% Glycerol, 0.01% Thimerosal.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX101929 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody (GTX101929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GOT2 antibody detects GOT2 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GOT2 antibody (GTX101929) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GOT2 antibody detects GOT2 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma tissue.
GOT2 antibody (GTX101929) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GOT2 antibody detects GOT2 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
10% SDS-PAGE
GOT2 antibody (GTX101929) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GOT2 antibody detects GOT2 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
GOT2 antibody (GTX101929) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunofluorescence analysis of methanol-fixed HeLa, using GOT2(GTX101929) antibody at 1:500 dilution.

GOT2 antibody detects GOT2 protein by western blot analysis.
A. 50 μg rat lung lysate/extract
10% SDS-PAGE
GOT2 antibody (GTX101929) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using GOT2(GTX101929) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min