Dengue virus NS4B protein antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Dengue virus NS4B protein antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX103349
• Supplier Catalog Number: GTX103349
• Alternative Catalog Number: GTX103349-100,GTX103349-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: FACS, ICC, WB
• Species Reactivity: Virus
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of Dengue virus NS4B protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: Dengue Virus Non-structural protein 4B , Dengue Virus NS4B , DENV Non-structural protein 4B , DENV NS4B

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 27
• Sensitivity: This antibody was raised against Dengue virus 2 NS4B protein, which may cross react with NS4B protein of Dengue virus 3.
• Buffer: 1XPBS (pH7), 40% Glycerol, 0.01% Thimerosal.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX103349 WB Image

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX103349) diluted at 1:2000.
Blue: Hoechst 33342 staining.

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extrac
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
12% SDS-PAGE
NS4B (Dengue virus) antibody (GTX103349) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NS4B (Dengue virus) antibody detects NS4B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS4B (Dengue virus) protein stained by NS4B (Dengue virus) antibody (GTX103349) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Sample(15 μg of cell lysate)
A: BHK21
B: DV infected BHK21
10% SDS PAGE
GTX103349 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX103349 IF Image Immunofluorescence analysis of Denque virus 2-infected BHK-21, using Non-structural protein 4B (Dengue virus 2 ) antibody (GTX103349) at 1:2000 dilution.