WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX105085 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
LOXL2 antibody detects LOXL2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human esophageal carcinoma.LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:3000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: LOXL2 protein stained by LOXL2 antibody (GTX105085) diluted at 1:500.. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:5000.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LOXL2 antibody (GTX105085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
LOXL2 antibody detects LOXL2 protein by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
LOXL2 antibody detects LOXL2 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.LOXL2 stained by LOXL2 antibody (GTX105085) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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