WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX111814 WB Image
Mre11 antibody [C1C3-2] detects Mre11 protein at nucleus by immunofluorescent analysis. Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Mre11 protein stained by Mre11 antibody [C1C3-2] (GTX111814) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Immunoprecipitation of Mre11 protein from HeLa whole cell extracts using 5 μg of Mre11 antibody [C1C3-2] (GTX111814). Western blot analysis was performed using Mre11 antibody [C1C3-2] (GTX111814). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody [C1C3-2] (GTX111814) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Mre11 antibody [C1C3-2] (GTX111814) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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