WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX111979 ICC/IF Image
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using FLJ32786(GTX111979) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FLJ32786 antibody [N2C1], Internal (GTX111979) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
FLJ32786 antibody [N2C1], Internal detects LRGUK protein by Western blot analysis. A. 30 μg Huh7 whole cell lysate/extract 7.5 % SDS-PAGE FLJ32786 antibody [N2C1], Internal (GTX111979) dilution: 1:1000
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FLJ32786 antibody [N2C1], Internal (GTX111979) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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