PRX III antibody [N1C3], Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: PRX III antibody [N1C3], Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX112004
• Supplier Catalog Number: GTX112004
• Alternative Catalog Number: GTX112004-100,GTX112004-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC-P, WB
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PRX III. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: peroxiredoxin 3 , AOP-1 , AOP1 , HBC189 , MER5 , PRO1748 , SP-22 , prx-III

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 28
• NCBI: 10935
• UniProt: P30048
• Buffer: 0.1M Tris-Glycine (pH7), 10% Glycerol, 0.01% Thimerosal.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX112004 IHC-P Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
C: Molt-4 (GTX27912)
D: Raji
12% SDS PAGE
GTX112004 diluted at 1:1000

Peroxiredoxin 3 antibody [N1C3] detects Peroxiredoxin 3 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: Peroxiredoxin 3 protein stained by Peroxiredoxin 3 antibody [N1C3] (GTX112004) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX112004 diluted at 1:1000

Wild-type (WT) and PRX III knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PRX III antibody [N1C3] (GTX112004) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.