WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX112287 WB Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using CRMP5(GTX112287) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CRMP5 antibody [N1C1] (GTX112287) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRMP5 antibody [N1C1] detects CRMP5 protein at cytoplasm in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. CRMP5 antibody [N1C1] (GTX112287) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
CRMP5 antibody [N1C1] detects CRMP5 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: CRMP5 protein stained by CRMP5 antibody [N1C1] (GTX112287) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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