WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX112361 IHC-P Image
Sample (30 μg of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE GTX112361 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GLUD1+2 antibody detects GLUD1+2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GLUD1+2 protein stained by GLUD1+2 antibody (GTX112361) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Sample (50 μg of whole cell lysate) A: mouse testis 7.5% SDS PAGE GTX112361 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GLUD1+2 antibody (GTX112361) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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