WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX112731 IHC-P Image
COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. COL1A1 antibody [N1N2], N-term (GTX112731) dilution: 1:500.
COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
Immunoprecipitation of COL1A1 protein from SK-N-AS whole cell extracts using 5 μg of COL1A1 antibody [N1N2], N-term (GTX112731). Western blot analysis was performed using COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
COL1A1 antibody detects COL1A1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody (GTX112731) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
COL1A1 antibody [N1N2], N-term detects secreted COL1A1 protein in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
COL1A1 antibody [N1N2], N-term detects COL1A1 protein at cytoplasm by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: COL1A1 stained by COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm.
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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