COL1A1 antibody [N1N2], N-term, IgG, Clone: Polyclonal, Unconjugated, Human

Catalog Number: GTX112731
Article Name: COL1A1 antibody [N1N2], N-term, IgG, Clone: Polyclonal, Unconjugated, Human
Biozol Catalog Number: GTX112731
Supplier Catalog Number: GTX112731
Alternative Catalog Number: GTX112731-100,GTX112731-25
Manufacturer: GeneTex
Host: Human
Category: Antikörper
Application: WB, IHC-P, ICC, IP
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human COL1A1. The exact sequence is proprietary.
Conjugation: Unconjugated
Alternative Names: collagen type I alpha 1 chain , EDSARTH1 , EDSC , OI1 , OI2 , OI3 , OI4
Clonality: Polyclonal
Concentration: 0.12 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation: Polyclonal
Molecular Weight: 139
Isotype: IgG
NCBI: 1277
UniProt: P02452
Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Purity: Purified by antigen-affinity chromatography.
COL1A1 antibody [N1N2], N-term detects COL1A1 protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COL1A1 stained by COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.
COL1A1 antibody [N1N2], N-term detects secreted COL1A1 protein in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
COL1A1 antibody detects COL1A1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody (GTX112731) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunoprecipitation of COL1A1 protein from SK-N-AS whole cell extracts using 5 μg of COL1A1 antibody [N1N2], N-term (GTX112731).
Western blot analysis was performed using COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
COL1A1 antibody [N1N2], N-term detects COL1A1 protein at secreted on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
COL1A1 antibody [N1N2], N-term detects secreted COL1A1 protein in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A1 antibody [N1N2], N-term (GTX112731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.