mtTFA antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: mtTFA antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX112760
• Supplier Catalog Number: GTX112760
• Alternative Catalog Number: GTX112760-100,GTX112760-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC-P, IP, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Full length human mtTFA Recombinant protein.
• Conjugation: Unconjugated
• Alternative Names: transcription factor A, mitochondrial , MTDPS15 , MTTF1 , MTTFA , TCF6 , TCF6L1 , TCF6L2 , TCF6L3

Product Properties

• Clonality: Polyclonal
• Concentration: 0.43 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 29
• NCBI: 7019
• UniProt: Q00059
• Buffer: 1XPBS pH7, 1% BSA, 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX112760 WB Image

mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.
Green: mtTFA protein stained by mtTFA antibody (GTX112760) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using mtTFA(GTX112760) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

mtTFA antibody immunoprecipitates mtTFA protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 μg of mtTFA antibody (GTX112760) 12% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody (GTX112760) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted at 1:1000.

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: mtTFA protein stained by mtTFA antibody (GTX112760) diluted at 1:500.
Red: Mitotracker, a mitochondria marker, diluted at 1:2000.
Blue: Hoechst 33342 staining.

TFAM antibody detects TFAM protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TFAM stained by TFAM antibody (GTX112760) diluted at 1:1000.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (GTX112760) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.