HDAC3 antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: HDAC3 antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX113303
• Supplier Catalog Number: GTX113303
• Alternative Catalog Number: GTX113303-100,GTX113303-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ChIP, ICC, IHC, IHC-P, IP, WB
• Species Reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: Recombinant protein encompassing a sequence within the center region of human HDAC3. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: histone deacetylase 3 , HD3 , KDAC3 , RPD3 , RPD3-2

Product Properties

• Clonality: Polyclonal
• Concentration: 0.32 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 49
• NCBI: 8841
• UniProt: O15379
• Buffer: 1XPBS (pH7), 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX113303 ICC/IF Image

HDAC3 antibody detects HDAC3 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted by 1:1000.

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HDAC3(GTX113303) antibody at 1:250 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 μg of either control rabbit IgG or anti-HDAC3 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HDAC3 antibody immunoprecipitates HDAC3 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 μg of HDAC3 antibody (GTX113303) 7.5% SDS-PAGE The immunoprecipitated HDAC3 protein was detected by HDAC3 antibody (GTX113303) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody (GTX113303) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.