ACADVL antibody [N1C1], Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: ACADVL antibody [N1C1], Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX114232
• Supplier Catalog Number: GTX114232
• Alternative Catalog Number: GTX114232-100,GTX114232-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ACADVL. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: acyl-CoA dehydrogenase very long chain , ACAD6 , LCACD , VLCAD

Product Properties

• Clonality: Polyclonal
• Concentration: 0.3 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 70
• NCBI: 37
• UniProt: P49748
• Buffer: 1XPBS (pH7), 1% BSA, 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX114232 IHC-P Image

Sample (50 μg of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX114232 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ACADVL antibody [N1C1] detects ACADVL protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human A549 xenograft.
ACADVL antibody [N1C1] (GTX114232) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody [N1C1] (GTX114232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Wild-type (WT) and ACADVL knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody [N1C1] (GTX114232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACADVL antibody [N1C1] (GTX114232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

ACADVL antibody [N1C1] detects ACADVL protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ACADVL stained by ACADVL antibody [N1C1] (GTX114232) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

ACADVL antibody [N1C1] detects ACADVL protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ACADVL stained by ACADVL antibody [N1C1] (GTX114232) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

ACADVL antibody [N1C1] detects ACADVL protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
ACADVL stained by ACADVL antibody [N1C1] (GTX114232) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min