WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX114439 IHC-Fr Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (GTX114439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GNAO1 antibody detects GNAO1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. GNAO1 antibody (GTX114439) dilution: 1:500.
GNAO1 antibody detects GNAO1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GNAO1 stained by GNAO1 antibody (GTX114439) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Immunoprecipitation of GNAO1 protein from HeLa whole cell extracts using 5 μg of GNAO1 antibody (GTX114439). Western blot analysis was performed using GNAO1 antibody (GTX114439). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GNAO1 antibody detects GNAO1 protein at cell membrane and cytoplasm in rat pancreas by immunohistochemical analysis. Sample: Paraffin-embedded rat pancreas. GNAO1 antibody (GTX114439) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (GTX114439) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GNAO1 antibody detects GNAO1 protein at cell membrane and cytoplasm in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. GNAO1 antibody (GTX114439) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (GTX114439) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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