Histone H1.0 antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Histone H1.0 antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX114462
• Supplier Catalog Number: GTX114462
• Alternative Catalog Number: GTX114462-100,GTX114462-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Histone H1.0. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: H1 histone family member 0 , H10 , H1FV

Product Properties

• Clonality: Polyclonal
• Concentration: 0.4 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 21
• NCBI: 3005
• UniProt: P07305
• Buffer: 1XPBS (pH7), 1% BSA, 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:1000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX114462 ICC/IF Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Histone H1.0(GTX114462) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Histone H1.0 antibody detects Histone H1.0 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H1.0 antibody (GTX114462) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using Histone H1.0(GTX114462) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Histone H1 antibody detects Histone H1 protein by western blot analysis. HepG2 whole cell extracts and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody (GTX114462) at a dilution of 1:10000 and developed with Trident femto Western HRP Substrate (GTX14698). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Histone H1.0 antibody detects Histone H1.0 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H1.0 antibody (GTX114462) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Histone H1.0 antibody detects Histone H1.0 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Histone H1.0 antibody (GTX114462) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Histone H1 antibody detects Histone H1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody (GTX114462) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1.0 antibody (GTX114462) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.