H-Ras antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: GTX116041
Article Name: H-Ras antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: GTX116041
Supplier Catalog Number: GTX116041
Alternative Catalog Number: GTX116041-100,GTX116041-25
Manufacturer: GeneTex
Host: Rabbit
Category: Antikörper
Application: ICC, IHC-P, IP, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human H-Ras. The exact sequence is proprietary.
Conjugation: Unconjugated
Alternative Names: HRas proto-oncogene, GTPase , C-BAS/HAS , C-H-RAS , C-HA-RAS1 , CTLO , H-RASIDX , HAMSV , HRAS1 , KRAS , KRAS2 , Ki-Ras , RASH1 , RASK2 , c-K-ras , c-Ki-ras , p21ras
Clonality: Polyclonal
Concentration: 1.87 mg/ml (Please refer to the vial label for the specific concentration.)
Molecular Weight: 21
Sensitivity: This antibody is specific for H-Ras protein, and it does not cross react with human N-Ras or K-Ras protein.
NCBI: 3265
UniProt: P01112
Buffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
Purity: Purified by antigen-affinity chromatography.
Form: Liquid
Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX116041 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with H-RAS antibody (GTX116041) diluted at 1:500 and competitor's antibody (SC-520) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with H-RAS antibody (GTX116041) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with H-RAS antibody (GTX116041) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using H-RAS (GTX116041) antibody at 1:200 dilution.
H-RAS antibody detects H-RAS protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
H-RAS antibody (GTX116041) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
H-RAS antibody detects H-RAS protein at membrane on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
H-RAS antibody (GTX116041) dilution: 1:500.
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Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min