Dengue virus NS2B protein antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Dengue virus NS2B protein antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX124246
• Supplier Catalog Number: GTX124246
• Alternative Catalog Number: GTX124246-100,GTX124246-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, WB
• Species Reactivity: Virus
• Immunogen: Full length Dengue virus 2 NS2B Recombinant protein (Dengue virus 2 (strain 16681 PDK 53))
• Conjugation: Unconjugated
• Alternative Names: Dengue Virus Non-structural protein 2B , Dengue Virus NS2B , DENV Non-structural protein 2B , DENV NS2B

Product Properties

• Clonality: Polyclonal
• Concentration: 1.15 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 15
• Sensitivity: This antibody was raised against Dengue virus 2 NS2B protein, which may cross react with NS2B protein of Dengue virus 3 but does not recignize with NS2B protein of JEV.
• Buffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX124246 WB Image

Non-infected (–) and infected (+) Huh-7 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus NS2B protein antibody (GTX124246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: Japanese encephalitis virus infect BHK-21
15% SDS PAGE
GTX124246 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.