Dengue virus NS5 protein antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number:
GTX124253
Supplier Catalog Number:
GTX124253
Alternative Catalog Number:
GTX124253-100,GTX124253-25
Manufacturer:
GeneTex
Host:
Rabbit
Category:
Antikörper
Application:
FACS, ICC, WB
Species Reactivity:
Virus
Immunogen:
Recombinant protein encompassing a sequence within the C-terminus region of Dengue virus NS5 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.
Conjugation:
Unconjugated
Alternative Names:
Dengue Virus Non-structural protein 5 , Dengue Virus NS5 , DENV Non-structural protein 5 , DENV NS5
Clonality:
Polyclonal
Concentration:
0.74 mg/ml (Please refer to the vial label for the specific concentration.)
Molecular Weight:
105
Sensitivity:
This antibody was raised against Dengue virus 2 NS5 protein, which may cross react with NS5 protein of Dengue virus 1/3/4 but does not recignize with NS5 protein of JEV.
Buffer:
1XPBS (pH7), 20% Glycerol, 0.025% ProClin 300.
Purity:
Purified by antigen-affinity chromatography.
Form:
Liquid
Application Notes:
WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX124253 WB Image
NS5 (Dengue virus) antibody detects NS5 (Dengue virus) protein at nucleus by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH. Green: NS5 (Dengue virus) protein stained by NS5 (Dengue virus) antibody (GTX124253) diluted at 1:2000. Blue: Hoechst 33342 staining.
Immunofluorescence analysis of paraformaldehyde-fixed BHK-21 cell, using NS5 (Dengue virus 2)(GTX124253) antibody at 1:2000 dilution.
Sample (20 μg of whole cell lysate) A: BHK-21 B: Dengue virus 2 infect BHK-21 C: JEV infect BHK-21 7.5% SDS PAGE GTX124253 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS5 protein antibody (GTX124253) diluted at 1:2000.
NS5 (Dengue virus) antibody detects NS5 (Dengue virus) protein at nucleus by immunofluorescent analysis. Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH. Green: NS5 (Dengue virus) protein stained by NS5 (Dengue virus) antibody (GTX124253) diluted at 1:2000. Blue: Hoechst 33342 staining.
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