Dengue virus NS1 protein antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Dengue virus NS1 protein antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX124280
• Supplier Catalog Number: GTX124280
• Alternative Catalog Number: GTX124280-100,GTX124280-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, FACS, ICC, IHC-P, IP, WB
• Species Reactivity: Virus
• Immunogen: Recombinant protein encompassing a sequence within the center region of Dengue virus NS1 protein (Dengue virus 2 (strain 16681 PDK 53)). The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: Dengue Virus Non-structural protein 1 , Dengue Virus NS1 , DENV Non-structural protein 1 , DENV NS1

Product Properties

• Clonality: Polyclonal
• Concentration: 0.25 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 40
• Sensitivity: This antibody was raised against Dengue virus 2 NS1 protein, which may cross react with NS1 protein of Dengue virus 1/3/4 but does not recignize with NS1 protein of JEV.
• Buffer: 1XPBS (pH7), 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX124280 WB Image

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (GTX124280) diluted at 1:2000.
Blue: Hoechst 33342 staining.

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 4 (right) were fixed in MeOH.
Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (GTX124280) diluted at 1:2000.
Blue: Hoechst 33342 staining.

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 1 (right) were fixed in MeOH.
Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (GTX124280) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Sample (20 μg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: JEV infect BHK-21
12% SDS PAGE
GTX124280 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

NS1 (Dengue virus) antibody detects NS1 (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS1 (Dengue virus) protein stained by NS1 (Dengue virus) antibody (GTX124280) diluted at 1:2000.
Blue: Hoechst 33342 staining.

NS1 (Dengue virus ) antibody detects NS1 (Dengue virus ) protein by Western blot analysis. Un-infected (-) and infected (+, DENV2 infection for ) BHK-21 whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NS1 (Dengue virus ) antibody (GTX124280) diluted by 1:2000.

Dengue virus NS1 protein antibody detects Dengue virus NS1 protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dengue virus BHK-21.
Green: Dengue virus NS1 protein stained by Dengue virus NS1 protein antibody (GTX124280) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Dengue virus NS1 protein antibody detects Dengue virus NS1 protein protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded BHK-21 infected with dengue virus.
Green: Dengue virus NS1 protein stained by Dengue virus NS1 protein antibody (GTX124280) diluted at 1:2000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min