Pcna antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Pcna antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX124496
• Supplier Catalog Number: GTX124496
• Alternative Catalog Number: GTX124496-100,GTX124496-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: IHC, IHC-Fr, IHC-P, WB
• Species Reactivity: Fish, Zebrafish
• Immunogen: The immunogen used to generate this antibody corresponds to zebrafish Pcna
• Conjugation: Unconjugated
• Alternative Names: proliferating cell nuclear antigen , cb16 , fa28e03 , fb36g03 , wu:fa28e03 , wu:fb36g03

Product Properties

• Clonality: Polyclonal
• Concentration: 0.41 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 29
• NCBI: 30678
• UniProt: Q9PTP1
• Buffer: 1XPBS pH7, 1% BSA, 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IHC-Wm: 1:50-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX124496 IHC-Wm Image

Immunohistochemical analysis of paraffin-embedded zebrafish kidney, using pcna (GTX124496) antibody at 1:300 dilution.

Pcna antibody detects Pcna protein on Medaka by whole mount immunohistochemical analysis.
Sample: 7 days-post-fertilization medaka embryo.
Pcna antibody (GTX124496) dilution: 1:100.

Pcna antibody detects Pcna protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Pcna antibody (GTX124496) dilution: 1:50.

PCNA antibody detects PCNA protein by western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PCNA antibody (GTX124496) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Pcna antibody detects Pcna protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Pcna antibody (GTX124496) dilution: 1:50.

Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Pcna antibody (GTX124496) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.