WB: 1:500-1:3000. IHC-Wm: 1:100-1:500. IHC: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX124660 IHC-Wm Image
Immunohistochemical analysis of agarose-embedded zebrafish embryo, using tp63 (GTX124660) antibody at 1:500 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)
Tp63 antibody detects Tp63 protein at nucleus on whole-mount zebrafish embryos by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. Tp63 antibody (GTX124660) dilution: 1:200.
Tp63 antibody detects Tp63 protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. Tp63 antibody (GTX124660) dilution: 1:100.
p63 antibody detects p63 protein by western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody (GTX124660) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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