Histone H2A.XS139ph (phospho Ser139) antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Histone H2A.XS139ph (phospho Ser139) antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX127342
• Supplier Catalog Number: GTX127342
• Alternative Catalog Number: GTX127342-100,GTX127342-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Zebrafish
• Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Ser139 of zebrafish Histone H2A.X. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: H2A histone family, member X , zgc:56329

Product Properties

• Clonality: Polyclonal
• Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 15
• NCBI: 394048
• UniProt: Q7ZUY3
• Buffer: 1XPBS pH7, 1% BSA, 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:2000. IHC-Wm: 1:100-1:500. IHC: 1:100-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX127342 IHC-P Image

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) dilution: 1:100.

H2afx antibody detects H2afx protein at nucleus on UV-irradiated whole-mount zebrafish embryos (under panel) by immunohistochemical analysis.
Sample: H2afx antibody (GTX127342) dilution: 1:2000.
Image provided with permission courtesy of Dr. James Amatruda.

Histone H2A.XS139ph (phospho Ser139) antibody detects Histone H2A.XS139ph (phospho Ser139) protein on UV-irradiated zebrafish embryos (right panel) by whole mount immunohistochemical analysis.
Samples: 24 hours-post-fertilization zebrafish embryo.
Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) dilution: 1:100.

Untreated (–) and treated (+) Zebrafish embryos extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H2A.XS139ph (phospho Ser139) antibody (GTX127342) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.