RAS antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: GTX132480
Article Name: RAS antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: GTX132480
Supplier Catalog Number: GTX132480
Alternative Catalog Number: GTX132480-100,GTX132480-25
Manufacturer: GeneTex
Host: Rabbit
Category: Antikörper
Application: IHC-P, WB
Species Reactivity: Frog, Goat, Human, Mouse, Rat, Rodent, Zebrafish
Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human KRAS. The exact sequence is proprietary.
Conjugation: Unconjugated
Clonality: Polyclonal
Concentration: 0.27 mg/ml (Please refer to the vial label for the specific concentration.)
Buffer: 1XPBS pH7, 1% BSA, 20% Glycerol, 0.025% ProClin 300.
Purity: Purified by antigen-affinity chromatography.
Form: Liquid
Application Notes: WB: 1:500-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX132480 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (GI: gastrointestinal)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Ras antibody detects Ras protein at cytoplasm in human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
Ras antibody (GTX132480) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Ras antibody detects Ras protein at cell membrane in rat urinary bladder by immunohistochemical analysis.
Sample: Paraffin-embedded rat urinary bladder.
Ras antibody (GTX132480) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min