WB: 1:500-1:10000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX132480 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (GI: gastrointestinal)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with RAS antibody (GTX132480) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Ras antibody detects Ras protein at cytoplasm in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Ras antibody (GTX132480) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Ras antibody detects Ras protein at cell membrane in rat urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded rat urinary bladder. Ras antibody (GTX132480) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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