Zika virus Envelope protein antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Zika virus Envelope protein antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX133314
• Supplier Catalog Number: GTX133314
• Alternative Catalog Number: GTX133314-100,GTX133314-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC-P, WB
• Species Reactivity: Virus
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of Zika virus Envelope protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: Zika virus Envelope , ZIKV Envelope

Product Properties

• Clonality: Polyclonal
• Concentration: 0.45 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 54
• Buffer: 1XPBS (pH7), 1% BSA, 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX133314 WB Image

Immunofluorescent analysis of arboviruses infected cells using Zika virus Envelope protein antibody (GTX133314).
Samples: EUROIMMUN Arboviral Fever Mosaic 2 slide (FR 2668-1010-1).
Green: Zika virus Envelope protein antibody (GTX133314) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Immunofluorescent analysis of non-infected and infected vero or BHK-21 cells using Zika virus Envelope protein antibody (GTX133314).
Green: Zika virus Envelope protein antibody (GTX133314) diluted at 1:4000.

Mock and infected Vero and BHK-21 whole cell extracts (20 μg) were separated by gradient gel, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133314) diluted at 1:4000.

Immunofluorescent analysis of Zika Virus-PRVABC59 infected (A) and non-infected (B) vero cells using Zika virus Envelope protein antibody (GTX133314).
Green: Zika virus Envelope protein antibody (GTX133314) diluted at 1:4000.

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133314) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-infected (–) and infected (+) vero cells (15 μg) were separated by gradient gel, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133314) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescent analysis of Zika virus infected cells using Zika virus NS5 protein antibody (GTX133314).
Sample: Mock and zika virus-infected cells.
Green: Zika virus Envelope protein antibody (GTX133314) diluted at 1:100.