Zika virus Envelope protein antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: Zika virus Envelope protein antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX133325
• Supplier Catalog Number: GTX133325
• Alternative Catalog Number: GTX133325-100,GTX133325-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, ICC, WB
• Species Reactivity: Virus
• Immunogen: Recombinant protein encompassing a sequence within the center region of Zika virus Envelope protein (Zika virus (strain H/PF/2013)). The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: Zika virus Envelope , ZIKV Envelope

Product Properties

• Clonality: Polyclonal
• Concentration: 1.14 mg/ml (Please refer to the vial label for the specific concentration.)
• Molecular Weight: 54
• Buffer: 1XPBS pH7, 20% Glycerol, 0.025% ProClin 300.
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. ELISA: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX133325 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634157 as capture antibody at concentration of 5 μg/mL and GTX133325 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Mock and infected Vero whole cell extracts were separated by gradient gel, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133325) diluted at 1:2500.

Immunofluorescent analysis of non-infected and infected vero cells using Zika virus Envelope protein antibody (GTX133325).
Green: Zika virus Envelope protein antibody (GTX133325) diluted at 1:500.

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133325) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Zika virus Envelope protein antibody (GTX133325) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.