SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active), Unconjugated

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active), Unconjugated
• Biozol Catalog Number: GTX136085-PRO
• Supplier Catalog Number: GTX136085-pro
• Alternative Catalog Number: GTX136085-PRO-100
• Manufacturer: GeneTex
• Category: Proteine/Peptide
• Application: Receptor Binding Assays, WB
• Species Reactivity: Virus
• Conjugation: Unconjugated
• Alternative Names: Alpha variant Spike, B.1.1.7 Spike, COVID-19 Alpha variant Spike, SARS-CoV-2 Alpha variant Spike, covid-19, sars-cov-2

Product Description

SARS-CoV-2 (COVID-19) Spike S1 Protein, B.1.1.7 / Alpha variant, His tag (active), Unconjugated

Product Properties

• Concentration: 1.14 mg/ml (Please refer to the vial label for the specific concentration.)
• Buffer: PBS, no preservative.
• Expression System: HEK293 cells
• Form: Liquid
• Sequence: SARS-CoV-2 Spike of QHD43416.1 (1-685 a.a) with mutations (deletion 69-70, deletion 144, N501Y, A570D, D614G, P681H) and His tag at the C-terminus

Images

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) (UK variant) (GTX136085-pro) and SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (13.72-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) (UK variant) (GTX136085-pro) and SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (13.72-10000 ng/mL). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) (UK variant) (GTX136085-pro) and SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (14-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) protein (GTX136085-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with the SARS-CoV-2 (COVID-19) Spike RBD antibody [HL257] (GTX635692) diluted at 1:5000.

1 μg of GTX136085-pro SARS-CoV-2 (COVID-19) Spike S1 (del69-70, del44, N501Y, A570D, D614G...) Protein, His tag (active) protein was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

GTX136085-pro Functional Assay Image