c-Kit (phospho Tyr823) antibody, Unconjugated, Rabbit, Polyclonal

Product Overview

• Article Name: c-Kit (phospho Tyr823) antibody, Unconjugated, Rabbit, Polyclonal
• Biozol Catalog Number: GTX25634
• Supplier Catalog Number: GTX25634
• Alternative Catalog Number: GTX25634-50
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, WB
• Species Reactivity: Aves, Human, Mouse
• Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Kit that contains tyrosine 823. The sequence is conserved in cow and dog.
• Conjugation: Unconjugated
• Alternative Names: KIT proto-oncogene, receptor tyrosine kinase , C-Kit , CD117 , MASTC , PBT , SCFR

Product Description

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known a

Product Properties

• Clonality: Polyclonal
• Concentration: Batch dependent (Please refer to the vial label for the specific concentration.)
• Clone Designation: Polyclonal
• Molecular Weight: 110
• Isotype: IgG
• NCBI: 3815
• UniProt: P10721
• Buffer: PBS pH7.3, 1mg/ml BSA, 50% Glycerol, 0.05% Sodium azide.
• Source: Human
• Purity: Purified by antigen-affinity chromatography
• Form: Liquid

Application Notes

• Application Notes: ICC/IF: 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX25634 WB Image

ICC/IF analysis of Jurkat cells treated with 250 ng/ml SCF for 5 minutes using GTX25634 c-Kit (phospho Tyr823) antibody. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minutes