Whole-cell lysate of 293T cells overexpressing SARS-CoV-2 spike S1 subunit (C-terminal His-tagged). The translated amino acid sequence is based on QHD43416.1 (a.a. 1-685). Lysate prepared in RIPA buffer with no reducing agents and is unboiled. Please see
Concentration:
1 mg/ml (Please refer to the vial label for the specific concentration.)
Buffer:
50 mM Tris-HCl (pH8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Triton X-100, 0.25% SDS, 1 mM PMSF, 5 µg/ml Leupeptin, 2 µg/ml Aprotinin, 1 µg/ml Pepstatin A, 0.025% ProClin 300.
Form:
Liquid
GTX535663 WB Image
SARS-CoV-2 (COVID-19) spike S1 293T overexpression cell lysates (GTX535663) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody (GTX115045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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