Iba1 antibody [HL22], Unconjugated, Rabbit, Monoclonal

Product Overview

• Article Name: Iba1 antibody [HL22], Unconjugated, Rabbit, Monoclonal
• Biozol Catalog Number: GTX635363
• Supplier Catalog Number: GTX635363
• Alternative Catalog Number: GTX635363-100,GTX635363-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ICC, IHC-Fr, IHC-P, WB
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Iba1. The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: allograft inflammatory factor 1 , AIF-1 , IBA1 , IRT-1 , IRT1

Product Description

Iba1 antibody detects Iba1, a protein widely used by neuroscientists as a microglial marker and to gauge microglial activation. It is also expressed in macrophages. Discrimination between these two cell populations can be achieved via double staining wit

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Clone Designation: [HL22]
• Molecular Weight: 17
• NCBI: 199
• UniProt: P55008
• Buffer: PBS, no Preservative.
• Purity: Affinity purified by Protein A.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX635363 WB Image

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:150.
Blue: Fluoroshield with DAPI (GTX30920).

Iba1 antibody [HL22] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Iba1 antibody [HL22] detects Iba1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Iba1 antibody detects Iba1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Iba1 stained by Iba1 antibody (GTX635363) diluted at 1:500.

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Iba1 antibody [HL22] (GTX635363) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse spinal cord.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).

Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse brain.
Green: Iba1 stained by Iba1 antibody [HL22] (GTX635363) diluted at 1:100.
Red: Iba1 stained by Iba1 antibody [HL22-RT] (GTX635400) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).