SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402], Unconjugated, Rabbit, Monoclonal

Product Overview

• Article Name: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402], Unconjugated, Rabbit, Monoclonal
• Biozol Catalog Number: GTX635713
• Supplier Catalog Number: GTX635713
• Alternative Catalog Number: GTX635713-100,GTX635713-25
• Manufacturer: GeneTex
• Host: Rabbit
• Category: Antikörper
• Application: ELISA, ICC, WB
• Species Reactivity: Virus
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of SARS-CoV-2 (COVID-19) Spike (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.
• Conjugation: Unconjugated
• Alternative Names: 2019-nCoV Spike, COVID-19 Spike, SARS-CoV-2 Spike, covid-19, sars-cov-2

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Clone Designation: [HL13402]
• Sensitivity: This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.
• Buffer: PBS, no Preservative.
• Purity: Affinity purified by Protein A.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX635713 ELISA Image

Sandwich ELISA detection of recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.7 alpha variant; B.1.351 beta variant; P.1 gamma variant; B1.617.2 delta variant) using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL)

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below._x000D__x000D_
Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL) _x000D_
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL).

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike ( L18F,..., K417N, E484K, N501Y,...)(ECD) Protein, His tag (active) (South Africa variant) (GTX136061-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike (L18F,..., K417T, E484K, N501Y, D614G,...,V987P)(ECD), His tag (active) (B.1.1.28) (GTX136091-pro) and SARS-CoV-2 (COVID-19) Spike (ECD) protein, His tag (active) (WT) (GTX135972-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (501.V1) spike protein, SARS-CoV-2 (501.V2) spike protein and SARS-CoV-2 spike protein (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 spike (trimer) protein (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect bound primary antibody.

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 spike (trimer) protein (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) (100-1.19*10^-5 μM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 0.19 μM

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL13402] (GTX635713) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.