Recombinant protein corresponding to SARS-CoV-2 (COVID-19) Spike RBD (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.
Conjugation:
Unconjugated
Alternative Names:
covid-19, sars-cov-2
Clonality:
Monoclonal
Concentration:
1 mg/ml (Please refer to the vial label for the specific concentration.)
Clone Designation:
[HL1003-HU]
Buffer:
PBS, no Preservative.
Purity:
Affinity purified by Protein A.
Form:
Liquid
Application Notes:
*Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX635866 ELISA Image
Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) as detection antibody at concentration of 1 μg/mL. Human IgG antibody (HRP) was diluted at 1:200000 and used to detect the primary antibody.
Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX135817-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) as detection antibody at concentration of 1 μg/mL. Human IgG antibody (HRP) was diluted at 1:200000 and used to detect the primary antibody.
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