CTCF antibody, Unconjugated, Rabbit, Polyclonal

Catalog Number: GTX65926
Article Name: CTCF antibody, Unconjugated, Rabbit, Polyclonal
Biozol Catalog Number: GTX65926
Supplier Catalog Number: GTX65926
Alternative Catalog Number: GTX65926-100
Manufacturer: GeneTex
Host: Rabbit
Category: Antikörper
Application: ChIP, ICC, IHC-P, IP, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: Recombinant funsion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).
Conjugation: Unconjugated
Alternative Names: CCCTC-binding factor , MRD21
This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA ta
Clonality: Polyclonal
Concentration: Batch dependent (Please refer to the vial label for the specific concentration.)
Clone Designation: Polyclonal
Molecular Weight: 83
Isotype: IgG
NCBI: 10664
UniProt: P49711
Buffer: PBS pH7.3, 50% Glycerol, 0.02% Sodium azide.
Source: Human
Purity: Purified by affinity chromatography
Form: Liquid
Application Notes: WB: 1:1000 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. IP: 1:50 - 1:100. ChIP assay: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
ChIP analysis of HCT116 cell lysate using GTX65926 CTCF antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
WB analysis of 293T cell lysate using GTX65926 CTCF antibody.
Dilution : 1:1000
Loading : 25μg per lane
IP analysis of HeLa cell lysate using GTX65926 CTCF antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000
ICC/IF analysis of HeLa cells using GTX65926 CTCF antibody.
Blue : DAPI
Dilution : 1:100
ICC/IF analysis of U2OS cells using GTX65926 CTCF antibody.
Blue : DAPI
Dilution : 1:100
GTX65926 IHC-P Image
IHC-P analysis of rat liver tissue using GTX65926 CTCF antibody.
Dilution : 1:200
IHC-P analysis of rat brain tissue using GTX65926 CTCF antibody.
Dilution : 1:200
IHC-P analysis of mouse brain tissue using GTX65926 CTCF antibody.
Dilution : 1:200