Nuclear Matrix Protein p84 antibody [5E10], IgG2b, Clone: [5.00E+10], Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: Nuclear Matrix Protein p84 antibody [5E10], IgG2b, Clone: [5.00E+10], Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: GTX70220
• Supplier Catalog Number: GTX70220
• Alternative Catalog Number: GTX70220-100
• Manufacturer: GeneTex
• Host: Mouse
• Category: Antikörper
• Application: ChIP, ICC, IHC, IHC-P, IP, WB
• Species Reactivity: Hamster, Human, Monkey, Mouse, Rat
• Immunogen: Amino acids 15-374 of human p84 expressed in E. coli.
• Conjugation: Unconjugated
• Alternative Names: THO complex 1 , HPR1 , P84 , P84N5

Product Description

Nuclear Matrix Protein p84 antibody detects THO complex subunit 1, which is an 84 kDa nuclear matrix protein often referred to as just ''p84. p84 is involved in mRNA transcription and is considered to be a nuclear marker. p84 antibody is an effective rea

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Clone Designation: [5.00E+10]
• Molecular Weight: 76
• Isotype: IgG2b
• Sensitivity: Knockdown/Knockout validation was supported by references (PMID:25296641)
• NCBI: 9984
• Pubmed: 27019227, 32747664,
• UniProt: Q96FV9
• Buffer: 1XPBS pH7, 20% Glycerol, no Preservative.
• Source: Human
• Purity: Protein G purified
• Form: Liquid

Application Notes

• Application Notes: WB: 0.3 - 2 µg/ml. ICC/IF: 0.5 - 2 µg/ml. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX70220 IHC-P Image

Sample (50 μg of whole cell lysate)
A: mouse Cerebellum
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

p84 antibody [5E10] immunoprecipitates p84 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 μg of pre-immune mouse IgG C : Immunoprecipitation of p84 by 3 μg of p84 antibody [5E10] (GTX70220) 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by p84 antibody [5E10] (GTX70220) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221667-01) was used as a secondary reagent.

Sample (30 μg of whole cell lysate)
A: HeLa
B: HeLa nucleus
7.5% SDS PAGE
GTX70220 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

Nuclear Matrix Protein p84 antibody [5E10] detects Nuclear Matrix Protein p84 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Nuclear Matrix Protein p84 stained by Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Detection of human p84/N5 in nuclear fraction by anti-p84/N5 5E10 monoclonal antibody (GTX70220) in western blot experiment. Lane 1: total lysate, Lane 2: cytoplasmic fraction, Lane 3: membrane fraction, Lane 4: nuclear fraction.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [5E10] (GTX70220) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.