Rad51 antibody [14B4], IgG2b, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: Rad51 antibody [14B4], IgG2b, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: GTX70230
• Supplier Catalog Number: GTX70230
• Alternative Catalog Number: GTX70230-100
• Manufacturer: GeneTex
• Host: Mouse
• Category: Antikörper
• Application: ICC, IHC, IHC-P, IP, PLA, WB
• Species Reactivity: Gallus, Human, Mouse, Rat
• Immunogen: Full length (amino acids 1-338) Rad51 expressed in E. coli.
• Conjugation: Unconjugated
• Alternative Names: RAD51 recombinase , BRCC5 , FANCR , HRAD51 , HsRad51 , HsT16930 , MRMV2 , RAD51A , RECA

Product Description

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This p

Product Properties

• Clonality: Monoclonal
• Concentration: 1 mg/ml (Please refer to the vial label for the specific concentration.)
• Clone Designation: [14B4]
• Molecular Weight: 37
• Isotype: IgG2b
• NCBI: 5888
• Pubmed: 25463395, 24847939,
• UniProt: Q06609
• Buffer: PBS, no preservative.
• Source: Human
• Purity: Purified by antigen-affinity chromatography.
• Form: Liquid

Application Notes

• Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX70230 ICC/IF Image

Mouse anti-RAD51 14B4 antibody (GTX70230) was used in IP assay (immunoprecipitation) using HeLa cell extract prepared with lysis 180 buffer (40 mM Tris-HCl pH8.0, 180 mM NaCl, 1 mMEDTA, 0.5% NP-40).
Rabbit anti-RAD51 antibody (GTX100469) was used for subsequent WB detection of immunoprecipated RAD51.
Whole cell extract was used as input.

RAD51 antibody 14B4 (GTX70230) detects RAD51 in nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
RAD51 antibody 14B4 (GTX702308) dilution: 1:200.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Rad51 antibody [14B4] (GTX70230) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.