CD147 antibody [9H5], IgG2b, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: CD147 antibody [9H5], IgG2b, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: GTX84801
• Supplier Catalog Number: GTX84801
• Alternative Catalog Number: GTX84801-100
• Manufacturer: GeneTex
• Host: Mouse
• Category: Antikörper
• Application: FACS, ICC, IHC-P, WB
• Species Reactivity: Canine, Human, Monkey, Mouse, Rat
• Immunogen: Full length human recombinant protein of human BSG(NP_001719) produced in HEK293T cell.
• Conjugation: Unconjugated
• Alternative Names: basigin (Ok blood group) , 5F7 , CD147 , EMMPRIN , EMPRIN , OK , SLC7A11 , TCSF

Product Description

Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8

Product Properties

• Clonality: Monoclonal
• Concentration: 0.64 mg/ml (Please refer to the vial label for the specific concentration.)
• Clone Designation: [9H5]
• Molecular Weight: 42
• Isotype: IgG2b
• NCBI: 682
• Pubmed: 33508487, 34172808,
• UniProt: P35613
• Buffer: PBS pH7.3, 1% BSA, 50% Glycerol, 0.02% Sodium azide.
• Source: Human
• Purity: Purified by affinity chromatography
• Form: Liquid

Application Notes

• Application Notes: WB: 1:200-1:1000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX84801 WB Image

WB analysis of various cell lines using GTX84801 CD147 antibody [9H5].
Loading : 35 ug per lane

WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84801 CD147 antibody [9H5].
Loading : 10 ug per lane
Dilution : 1:500

ICC/IF analysis of HT29 cells using GTX84801 CD147 antibody [9H5].

IHC-P analysis of human prostate tissue using GTX84801 CD147 antibody [9H5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

IHC-P analysis of human kidney tissue using GTX84801 CD147 antibody [9H5].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

WB analysis of HEK293T cells transfected with CD147 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84801 CD147 antibody [9H5].
Loading : 5 ug per lane

FACS analysis of HEK293T cells transfected with either CD147 plasmid(Red) or empty vector control plasmid(Blue) using GTX84801 CD147 antibody [9H5].

ICC/IF analysis of COS7 cells transiently transfected with CD147 plasmid using GTX84801 CD147 antibody [9H5].