ATP5B antibody [7F4], IgG1, Unconjugated, Mouse, Monoclonal

Product Overview

• Article Name: ATP5B antibody [7F4], IgG1, Unconjugated, Mouse, Monoclonal
• Biozol Catalog Number: GTX84843
• Supplier Catalog Number: GTX84843
• Alternative Catalog Number: GTX84843-100
• Manufacturer: GeneTex
• Host: Mouse
• Category: Antikörper
• Application: FACS, ICC, IHC-P, WB
• Species Reactivity: Canine, Human, Monkey
• Immunogen: Full length human recombinant protein of human ATP5B (NP_001677) produced in HEK293T cell.
• Conjugation: Unconjugated
• Alternative Names: ATP synthase F1 subunit beta , ATP5B , ATPMB , ATPSB , HEL-S-271

Product Description

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of

Product Properties

• Clonality: Monoclonal
• Concentration: 0.63 mg/ml (Please refer to the vial label for the specific concentration.)
• Clone Designation: [7F4]
• Molecular Weight: 57
• Isotype: IgG1
• NCBI: 506
• UniProt: P06576
• Buffer: PBS pH7.3, 1% BSA, 50% Glycerol, 0.02% Sodium azide.
• Source: Human
• Purity: Purified by affinity chromatography
• Form: Liquid

Application Notes

• Application Notes: WB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

Images

GTX84843 FACS Image

ICC/IF analysis of COS7 cells transiently transfected with ATP5B plasmid using GTX84843 ATP5B antibody [7F4].

IHC-P analysis of liver carcinoma tissue using GTX84843 ATP5B antibody [7F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

IHC-P analysis of breast adenocarcinoma tissue using GTX84843 ATP5B antibody [7F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

IHC-P analysis of endometrium adenocarcinoma tissue using GTX84843 ATP5B antibody [7F4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

WB analysis of HEK293T cells transfected with ATP5B plasmid (Right) or empty vector (Left) for 48 hrs using GTX84843 ATP5B antibody [7F4].
Loading : 5 ug per lane

FACS analysis of Jurkat cells using GTX84843 ATP5B antibody [7F4].
Red : Primary antibody
Blue : Negative control antibody

WB analysis of various cell lines using GTX84843 ATP5B antibody [7F4].
Loading : 35 ug per lane

ICC/IF analysis of HepG2 cells using GTX84843 ATP5B antibody [7F4].