Anti-Mouse IgG for IP, AlpSdAbs VHH(HRP) is designed for detecting the non-reduced mouse IgG(subtypes:IgG1, IgG2a, IgG2b, IgG3). Anti-Mouse IgG for IP, AlpSdAbs(TM) VHH(HRP) is based on Monovalent, recombinant single domain antibody to mouse IgG coupled to HRP, and the Anti-Mouse IgG for IP, AlpSdAbs(TM) VHH(HRP) detects the non-reduced form of mouse IgG (IgG1, IgG2a, IgG2b, IgG3) selectively, no reactivity with the reduced, SDS-denatured forms. When performing immunopreciptiation (IP) followed by western blotting, using Anti-mouse IgG for IP, AlpSdAbs(TM) VHH(HRP) to detect the primary antibody, only native antibody can be staining, not denatured heavy and light chains. High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production . . . .
WB: 1:5,000-1:10,000,,Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
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